Anti-AQP11 antibody detects AQP11 protein in mouse brain tissue.

The function of AQP11, the latest member of the aquaporin family, is not well understood. However, genetic complementation analysis revealed that loss of AQP11 due to a Cys227Ser mutation results in severe proximal tubule (PT) damage and renal failure in aqp11sjds/sjds mice, suggesting that the cysteine residues play a role in regulating AQP11 oligomeric state.

Using aqp11-specific antibodies, we were able to show that Anti-AQP11 antibody is expressed on the surface of capillary endothelium in the mouse brain. Furthermore, we found that AQP11 expression is regulated during postnatal development in the brain: at the stages of P1 and P7, it is mainly expressed on the surface of neurons; at P14, it is strongly induced in the brain parenchyma.

Anti-AQP11 Antibody: Properties and Benefits in Research

In the retina of the horse, AQP11 is also localized in the capillary endothelium extending from Muller cell bodies to the outer limiting membrane formed by Muller cell endfeet (Fig. 1d). The specificity of this detection was substantiated by preincubation of the antibody with the immunization peptide, which dose dependently abolished binding to AQP11 (Fig. 1e). Quantification of Western blot signals was performed using ImageQuantTL software (GE Healthcare, Freiburg, Germany). Results were normalized to beta actin and analyzed by Student s t test.